RNA Analytical Quality/Resources

1. High-Throughput Characterization Of RNA 5/9/2022

   How a multi-capillary electrophoresis (CE) system is used to efficiently separate a wide range of RNA-based molecules by size and demonstrates the multiplex capability for RNA characterization.

2. Sequencing Of Synthetic Oligonucleotides And Their Impurities 9/20/2022

   We demonstrate an automated, compliance-ready liquid chromatography-mass spectrometry (LC-MS) workflow for sequence confirmation of oligonucleotides and their impurities.

3. Methods for mRNA Poly(A) Sizing 4/26/2024

   This study used Oxford Nanopore Technologies' Direct RNA Sequencing Kit and obtained successful sizing of poly(A) tail regions. The results are presented in a histogram and table format.

4. Assessing Doggybone DNA For In Vitro Transcription 4/26/2024

   In this study, we evaluated the performance of two dbDNA IVT templates, each encoding the same mRNA (firefly luciferase or Fluc), by looking at the mRNA purity and in vivo potency of the mRNA IVT’d from each template.

5. A&M STABTEST Expands Service Offerings In Protein And Oligonucleotide Analysis With LC-MS System 2/22/2022

   CRO upgrades systems to provide regulatory compliance for large-molecule protein analysis, expanding their oligonucleotides capabilities.

6. Lipid Nanoparticle Compositional Analysis By UHPLC-CAD 6/30/2023

   Explore results generated from an internal study using a proprietary mRNA-LNP formulation and UHPLC-CAD, a powerful technique for the analysis of compounds that do not contain chromophores.

7. Extending The Lower Limits Of Quantification Of A Therapeutic Oligonucleotide Through Microflow LC-MS/MS 3/4/2022

   Here a solution is presented to widely recognized challenges with ionpairing reversed phase liquid chromatography (IP-RP LC) through a novel microflow LC-MS strategy.

8. Metabolite ID And Relative Quantification Of Oligonucleotides In Plasma 3/4/2022

   This technical note describes the identification, relative quantification, and structural confirmation of the chain-shortened metabolites of a phosphorothioated oligonucleotide.

9. Future-Proofing mRNA Drug Manufacturing: The Value of Continuous Improvement 4/30/2024

   With so many uncertainties permeating the mRNA market, this largely preclinical industry is still seeking answers on how to reach successful therapeutic development. Identifying optimizations, both sequence-dependent and otherwise, to enhance the manufacturing process is crucial for advancing mRNA product development, which holds vast potential to address a wide range of indications.

10. Study Viral Infection In Resting Human CD4+ T Cells 11/28/2023

    Explore a study proving how synthetic single guide RNAs were used to optimize the editing conditions for resting human CD4+ T cells and maintain high cell viability for several weeks post Nucleofection.