High-Sensitivity IVT mRNA Analysis Using The Agilent Fragment Analyzer Systems

In the realm of biotherapeutics, particularly vaccine development, the quality analysis of in vitro transcribed (IVT) mRNA is paramount. The Agilent Fragment Analyzer systems, equipped with the High-Sensitivity (HS) RNA kit, have made significant strides in this area with optimized methods tailored for IVT mRNA assessment. These enhancements focus on critical quality attributes (CQAs) such as size, integrity, and purity to ensure that researchers can achieve reliable results for mRNA therapeutic applications.

The HS RNA kit features two primary methods: Method A for low-concentration samples (500 to 2,500 pg/µL) and Method B for mid-range concentrations (2,500 to 10,000 pg/µL). Both methods effectively analyze IVT mRNAs ranging from 200 to 6,000 nt, while newly developed extended methods (AE and BE) cater to longer RNA fragments up to 9,000 nt. Rigorous testing demonstrated that both methods maintain high accuracy and precision, with minimal variability across different concentrations.

With detection limits down to 15 pg/µL and robust performance across various sample sizes and concentrations, this innovative system stands as a vital tool in advancing mRNA research and therapeutic development. Discover how these advancements can enhance your mRNA analysis by accessing the full application note.