How To Navigate Through The Jungle Of Columns?

Oligonucleotides present unique chromatographic hurdles due to their high charge density and polarity. Because slight variations in length or side chain modifications can dramatically alter results, selecting the precise stationary phase is critical for data integrity. Whether you prioritize high-throughput robustness or the detailed flexibility required for R&D, the choice depends on specific workflow demands—ranging from sample volume to MS compatibility.

For purification, balancing resolution against loading capacity drives the decision between IP-RP and Anion Exchange methods. Meanwhile, the debate regarding bioinert versus stainless steel hardware continues, often hinging on the speed of analysis rather than strict necessity. In Part 6 of our 9-part Oligos Made Easy series, we provide decision trees to help navigate these technical trade-offs. Read the full article to help ensure your column selection aligns perfectly with your analytical goals.