RNA Analytical Quality/Resources

  1. Data Processing For Mass Confirmation, Impurities Analysis Of Oligos 5/5/2022

    Explore an automated, compliance-ready LC-MS workflow for intact mass confirmation and purity analysis of synthetic oligonucleotides including siRNA and large single guide RNA oligonucleotides.

  2. Metabolite ID And Relative Quantification Of Oligonucleotides In Plasma 3/4/2022

    This technical note describes the identification, relative quantification, and structural confirmation of the chain-shortened metabolites of a phosphorothioated oligonucleotide.

  3. Optimizing mRNA For Success: A Guide To Enhanced Stability, Delivery, And Efficacy 6/11/2024

    RNA offers exciting potential for vaccines, gene therapies, and personalized medicine, but challenges related to stability, delivery, and manufacturing persist.

  4. Quantification Of Large Oligonucleotides Using High Resolution MS/MS 3/4/2022

    Explore how to achieve sensitive, high-resolution analysis of large oligonucleotides, with the opportunity to perform both qualitative and quantitative analysis in a single run.

  5. Optimizing qPCR For Broad-Range Transcript Detection In Anti-PD-1 Therapy 4/23/2025

    Optimize qPCR protocols for greater sensitivity and reproducibility to accurately measure immune-related gene expression and better identify patients who will benefit from anti-PD-1 immunotherapy.

  6. Exploring Osmolality As A CQA Within RNA Therapeutics 10/3/2023

    Discover how osmolality can be applied throughout the bioprocessing workflow for RNA, and recognize the importance of this sensitive measurement, from formulation optimization to cryopreservation techniques.

  7. CRISPR sgRNA For Successful Gene Editing: Discovery To Clinic 9/25/2023

    The single guide RNA (sgRNA) is one of the key components of successful CRISPR gene editing. Here we cover all the important aspects of sgRNA and Synthego’s sgRNA products.

  8. Five Essentials For Accurate Oligonucleotide Chemistry 3/4/2024

    Explore five key areas in oligonucleotide chemistry where improvements will have a huge influence on your synthesis quality.

  9. Novel Plasmid DNA-Encoded Poly(A) Tails For mRNA Synthesis 4/26/2024

    Here, we evaluate the stability of these variant poly(A) regions and the biological activity of the mRNA containing the variant poly(A) tails made from these templates.

  10. Streamlining The Importing & Exporting Of Biological Samples 9/1/2024

    Create a seamless and predictable import and export process by working with experienced bioanalytical laboratory partners, understanding what paperwork samples require, and proactive planning.