RNA Manufacturing Resources

1. Extending The Lower Limits Of Quantification Of A Therapeutic Oligonucleotide Through Microflow LC-MS/MS 3/4/2022

   Here a solution is presented to widely recognized challenges with ionpairing reversed phase liquid chromatography (IP-RP LC) through a novel microflow LC-MS strategy.

2. Flexibility And High-Quality Extraction Of Genomic DNA And RNA From Yeast, Saccharomyces Cerevisiae 4/29/2022

   In this report, we analyze the quality and quantity of gDNA as well as RNA from S. cerevisiae with Pall NAB Nanosep centrifugal devices in comparison with a renowned commercial brand.

3. Adenovirus Production In Single-Use Bioreactor System 3/8/2019

   Rocking bioreactor systems can shorten the seed train prior to inoculation and provide optimized growth conditions for sensitive cells. This study presents a robust production of adenovirus using such a system.

4. Multivariate Inter-Stage Batch Profile Monitoring And Future State Estimation 2/3/2022

   How much quicker and easier would it be if we could know the process’s health by looking at a single metric rather than checking hundreds of parameters?

5. Residual Plasmid Quantification In Gene Therapy Manufacturing Workflows 6/7/2024

   This article details the design and application of a Plasmid DNA - Kanamycin Resistance Gene (pDNA-KanR) kit for residual plasmid quantification in gene therapy manufacturing.

6. Ultra-Sensitive Assay For Anti-Sense Oligonucleotide Quantification 3/1/2022

   Learn about a new level in sensitivity for challenging analytes in matrix that opens doors for accurate bioanalysis and metabolism studies with specificity not achievable with orthogonal methods.

7. Novel Lipid Nanoparticle Delivery Reagent And Manufacturing Workflow 4/18/2022

   We aim to highlight that commercially available LNP reagent mix, GenVoy-ILM, is an accessible and easy-to-use LNP formulation that allows for rapid preclinical development of RNA vaccines.

8. Navigating The Regulatory Maze: Fundamentals For Drug Development 7/15/2022

   Learn about key regulatory concepts and ways to accelerate and reduce risk throughout the drug development journey. 

9. How Lipid Nanoparticles Enable Next-Gen Delivery 10/20/2025

   Cell therapy’s future depends on smarter gene delivery. Learn how lipid nanoparticles offer a scalable, cell-friendly alternative to electroporation and viral vectors for manufacturing efficiency.

10. Where Can Novel Non-Viral Polymeric Delivery Agents Take Us? 1/18/2023

    Explore ongoing work to harness different types of HDR templates in order to build cutting-edge systems with the potential to enable point-of-care cell and gene therapy manufacture.