RNA Manufacturing Resources

1. Developing An mRNA-Encoded Antibody Platform To Accelerate Therapies To Clinic 11/20/2024

   In this paper, we present the foundation of an mRNA-LNP platform for encoding and expressing therapeutic antibodies in vivo, eliminating the need for costly and time-consuming manufacturing.

2. Scale-Up And Manufacturing Of Self-Amplifying RNA-LNPs 4/18/2022

   This poster shows the work to scale up COVID-19 self-amplifying RNA-Lipid nanoparticle using PNI developed ionizable lipid and formulation from 0.2, to 50 mg scale using PNI’s Manufacturing Platform.

3. Manufacturing Challenges With High Concentration Biologics 3/18/2024

   Explore key process operations, some of the challenges in manufacturing high-concentration biologics, and factors to consider for process optimization.

4. mRNA-Lipid Nanoparticles Circumvent Viral Vector Limitations 6/1/2022

   Both physical and chemical nonviral gene delivery systems for ex vivo genetic modification offer advantages over viral vectors, including smaller scale production and the low risk of immunogenicity.

5. New To mRNA Manufacturing? Considerations For Starting 6/15/2022

   Explore considerations for starting mRNA manufacturing related to the following topics: DNA template, mRNA product - IVT, mRNA product - purification, LNP encapsulation, and fill-finish.

6. RNA: The Next Era In Therapeutic Development 7/26/2023

   Review the unique set of challenges for RNA production that must be considered and solved to reach their full therapeutic potential.

7. Accelerated Development Of Self-Amplifying mRNA (saRNA) Vaccines Using Microfluidics 4/14/2022

   We showcased the utility of microfluidics to enable low shear, rapid screening of preclinical candidates and the swift advancement to GMP-enabling studies.

8. Five Essentials For Accurate Oligonucleotide Chemistry 3/4/2024

   Explore five key areas in oligonucleotide chemistry where improvements will have a huge influence on your synthesis quality.

9. Developing Robust mRNA Processes In A cGMP Environment 2/17/2023

   Explore current and emerging technologies to optimize mRNA manufacturing, especially when considering commercial production and the validation of processes in a cGMP environment.

10. Standardize Scale-Up And Reduce Time To Market With Mixing Cartridges 7/21/2023

    Despite the momentum of the RNA-LNP therapeutic market, significant manufacturing challenges remain. However, the right mixing could solve three of these key challenges regarding instrumentation.