RNA Formulation Resources

1. LNP Formulation For mRNA Delivery 2/15/2023

   Microfluidic mixing is one of the most effective means to formulate LNPs for its scalability and reproducibility. Explore LNP formulation, polishing, sterile filtration of mRNA-LNPs, and more.

2. Genome Editing Using mRNA-Lipid Nanoparticles For CAR T Cell Therapy 11/22/2023

   Gene delivery platforms must support a diversity of genetic engineering strategies for genomic medicine development. Learn how lipid nanoparticles can accelerate development from discovery to scale-up.

3. Versatile Lipid Nanoparticle Platform For Efficient Gene Editing 6/3/2025

   Lipid nanoparticles enable efficient gene editing in hard-to-transfect cells like T-cells and blood stem cells, which offers scalable, clinically relevant methods for developing advanced therapies for cancer and rare diseases.

4. Unpacking The Importance Of Lipid Nanoparticle Production Platforms 4/15/2024

   Batch processing platforms for LNPs lack efficiency, appropriate controls, and yield optimization. Continuous manufacturing platforms may address some of those issues.

5. Purification And QC Of An RNA 21‑Mer Oligonucleotide Via LC‑MS 1/27/2026

   Learn how buffer choice, gradient strategy, and pretreatment steps influence recovery, purity, and resolution when purifying a native RNA oligo, with analytical confirmation and practical guidance.

6. Scale-Up And Manufacturing Of Self-Amplifying RNA-LNPs 4/18/2022

   This poster shows the work to scale up COVID-19 self-amplifying RNA-Lipid nanoparticle using PNI developed ionizable lipid and formulation from 0.2, to 50 mg scale using PNI’s Manufacturing Platform.

7. Enabling Continuous Manufacturing For CAR-T And LNP-Based Therapies 6/27/2025

   Breakthrough therapies are challenged by complex, batch-based manufacturing. A novel, integrated system enables continuous processing, improving consistency and reducing manual handling for advanced modalities.

8. Freezing mRNA: Best Practice 7/14/2023

   By following some of the best practices in freezing mRNA, you can effectively balance product quality, cost-effectiveness, process safety, and efficiency.

9. Analysis Of LNP Lipids With Evaporative Light Scattering Detection 12/6/2024

   Jump into this thorough analysis of lipids in LNP formulations using HPLC, a technique crucial for quality control in the production of mRNA vaccines and therapeutics.

10. CQA Assessment Of LNP-Encapsulated IVT mRNA 4/21/2025

    Learn more about issued guidelines on Critical Quality Attributes for IVT mRNA vaccines and biotherapeutics to focus on drug substance and LNP-encapsulated drug product assessment using an analyzer system.