RNA Analytical/Quality Videos

1. Select An Ion-Pairing Agent For Impurity Analysis Of Oligonucleotides 5/4/2022

   Learn about method development considerations when selecting an appropriate ion-pairing agent and impurity analysis of dye-conjugated oligonucleotides.

2. Determining Fragment Length for Enzymatic Ligation of gRNA 4/29/2026

   Advancing RNA Live panelists Max De Long, Venkat Krishnamurthy and April Sena address two audience questions about the number/length of fragments for enzymatic ligation of gRNA.  

3. How Should We Better Interrogate gRNA Design Today? 4/29/2026

   Advancing RNA Live speaker April Sena emphasizes the need for a collaborative, public safety dataset for chemical modifications to streamline regulatory approvals, while Max De Long points out a potential drug clearance conundrum as we continue to improve our gRNA’s durability and stability. 

4. The Biggest Challenges Of Working With Ligase For siRNA Production 3/27/2026

   ALMAC expert Jill Caswell identifies the most overlooked challenges of working with ligase for the chemoenzymatic reaction, as well as the impact this is having today on enzyme engineering and purification. 

5. Bridging the Gap Between Analytical Control & Translation 6/9/2026

   Though we may have increasingly clear understandings of our mRNA-LNP products structurally, understanding our products’ cellular function remains challenging without extensive clinical data. Here, this Advancing RNA panel featuring AbbVie’s Elaine Peters, Tessera’s Ilya Shestopalov, and Sanofi’s Philippe Talaga addresses what innovation — both in research and method development — can help us get more functional information about our molecules as we wait for more clinical development.  

6. The Conjugation Conundrum: The Realities Of Conjugated LNP Manufacturing 2/24/2026

   In this Advancing RNA Live clip, CMC Consultant Sujit Jain explains the numerous ways that adding targeting ligands “stress tests” the standard LNP manufacturing platform, as well as outlines which capabilities are most important for companies to consider when selecting an outsourcing partner for their active delivery strategies.

7. Advancing Peptide Characterization Of Critical Quality Attributes With MMS 11/12/2025

   Axxium uncovered hidden peptide aggregation using MMS technology, revealing process-related potency issues missed by conventional methods. Learn how advanced structural analysis can strengthen quality control and biologics development.

8. The "Golden Idea Of The Platform" & Its Role In mRNA PD 8/22/2024

   Explore how early-stage companies can implement design space and analytical principles that will help us determine the biggest differences between constructs and how close we may be to a true “platform process.”

9. Beyond Cas9: How Diverse Nucleases are Redefining gRNA Design 4/29/2026

   As CRISPR technology evolves beyond the original Cas9, gRNAs are transitioning from simple targeting tools into highly engineered, complex molecules. In this Advancing RNA Live segment, speakers April Sena, Max De Long, and Venkat Krishnamurthy discuss how the rise of diverse nucleases — including prime editors — is impacting gRNA synthesis and purity.

10. mRNA/saRNA Manufacturing: Accelerate The Path To Clinic 3/22/2021

    Discover key upstream and downstream considerations for process development for RNA drug substance manufacturing and the parameters that need construct-specific optimization to increase yield and reduce impurities.