RNA Analytical/Quality Videos

  1. Development Of A Mitra Tip Extraction Assay Coupled With LC-MSMS 1/15/2025

    Watch this rundown of a novel method for quantifying L-Citrulline, L-Arginine, and L-Argininosuccinic acid using Mitra tip extraction coupled with LC-MS/MS for enhanced accuracy and reproducibility.

  2. Fermentation, Analytics, & Quality: Plasmid Production Advancements & Challenges for mRNA Development 4/11/2025

    Advancing RNA Live panelists Tyler Goodwin and April Sena start off our latest discussion on technology innovations in mRNA production by surveying the current manufacturing process for plasmid production, highlighting where they’ve seen advancements and what biotechs need to consider to obtain plasmids of the appropriate quality for mRNA production.

  3. The Conjugation Conundrum: The Realities Of Conjugated LNP Manufacturing 2/24/2026

    In this Advancing RNA Live clip, CMC Consultant Sujit Jain explains the numerous ways that adding targeting ligands “stress tests” the standard LNP manufacturing platform, as well as outlines which capabilities are most important for companies to consider when selecting an outsourcing partner for their active delivery strategies.

  4. Characterizing The Poly(A) Tail In mRNA Using Ion-Pairing 10/29/2026

    Digesting mRNA to isolate its polyA tail makes mass spectrometric analysis tractable, yet even after digestion the tail resolves into a heterogeneous mixture of lengths requiring careful control.

  5. Closing the Gap: How NIST is Addressing gRNA Quality 4/29/2026

    Life Edit Therapeutics’ April Sena provides an update on NIST’s gene editing consortium, teasing forthcoming deliverables from a working group focused on gRNA quality. 

  6. How Can Sponsors & CDMOs Collaborate On RNA Analytical/Process Development? 8/22/2024

    Having worked on both the innovator and CDMO sides of the industry, Advancing RNA LIVE panelist Khaled Yamout outlines where he sees the greatest weaknesses in these critical sponsor-CDMO partnerships and offers guidance.

  7. Validation Of mRNA Concentration Determination 8/20/2023

    mRNA-based therapeutics require comprehensive analytical testing to meet regulatory requirements. Optimize processes and ensure fast and reliable results as you bring your product to market.

  8. Analyzing COVID-19 Vaccine Purity Using LC-MS Technologies 11/8/2022

    Explore why process-related impurities are a concern in vaccines and present results and learnings from LC-MS analyses of several mRNA- and protein-based vaccines for COVID-19.

  9. The Current State of CDMO Platforms For gRNA Production 4/29/2026

    Max De Long, April Sena, and Venkat Krishnamurthy share their perspectives on the current “health” of CDMO SPOS and enzymatic ligation platforms, while also highlighting the “red flags” to look out for when taking stock of a CDMOs capabilities. 

  10. Engaging Early In Process Development To Bring mRNA Sequences To Life

    Examine why template sequence characteristics are relevant for cGMP manufacturing of high-quality mRNA and consider insights on how to develop a scalable, robust, and economically viable process.