Twist Of Two: 2D-LC For Oligos

Standard one-dimensional separation often hits a wall when facing complex impurities like truncated sequences or structural variants. Two-dimensional liquid chromatography (2D-LC) breaks through these limitations by coupling distinct chromatographic modes, effectively resolving co-eluting compounds that simpler methods cannot untangle. As the ninth and final installment of the Oligos Made Easy series, this overview explores the mechanics of orthogonality and the strategic trade-offs between Heart-Cut and Comprehensive Transfer workflows.

Whether the goal is detailed impurity profiling for R&D or targeted quality control, success relies on mastering the delicate balance of valve timing, column chemistry, and mobile phase compatibility. Read the full article to understand how to configure a setup that delivers the high-resolution insights necessary for modern biopharmaceutical characterization.