Purification And Quality Control Of An RNA 21-Mer Oligonucleotide Using Capto Q ImpRes And LC-MS

By Justin Townsend, Linda Hagman, Filip Bergqvist, Simon Åberg, Ganesh Nawale, Per Denker, and Peter Guterstam

Purifying therapeutic oligonucleotides demands methods that deliver high recovery, high purity, and reliable scalability. Gain insight into a complete workflow for isolating a native 21‑mer RNA oligo using strong anion exchange chromatography, highlighting how buffer selection, salt gradients, and sample pretreatment shape overall performance. Learn how optimized loading conditions and careful control of pH, conductivity, and additives such as urea or glycerol influence elution behavior and minimize undesired interactions. Analytical insights from both IEX‑HPLC and LC‑MS confirm the achievable purity levels and characterize common product‑related impurities.

Explore the full poster to see the complete data set and process recommendations for developing robust RNA purification strategies.