Oligonucleotide Purification And Synthesis

Efficient purification is essential to producing high‑quality oligonucleotides, especially as therapeutic developers demand tighter control over sequence integrity and impurity removal. Walk through the synthesis of a 21‑mer DNA oligo and the systematic optimization of a single‑step anion‑exchange purification method. Three resin candidates were evaluated for purity, binding capacity, and elution behavior, leading to clear differentiation between resin performance and selection of the most effective option. The optimized method was then verified at bench scale and successfully scaled to pilot scale while maintaining strong recovery and high purity.

Gain clarity on how subtle differences in resin chemistry, loading, and gradient design influence oligonucleotide separation, along with practical considerations for scaling up purification workflows. Explore the full application note for guidance on developing robust, chromatography‑based oligo purification processes.