HPLC Analysis Of Lipids Used In LNP Formulations With Evaporative Light Scattering Detection

Lipid nanoparticles (LNPs) are vital for the delivery of mRNA vaccines and hold immense potential for treating various diseases. This application note focuses on the development of HPLC methods for the analysis of LNPs, specifically targeting the identification and quantification of lipids using an evaporative light scattering detector (ELSD). Through this study, we investigate different column types, elution modes, and modifier suitability for achieving optimal separation and detection sensitivity.

By combining acetonitrile and methanol as eluents and incorporating ammonium acetate as a modifier, the research achieves excellent peak shapes and complete lipid elution. The optimized isocratic method minimizes analysis time, while the gradient method allows for the separation of potential impurities. The ELSD demonstrates high sensitivity, achieving LODs ranging from 0.8 to 3 µg/ml (4 to 15 ng) and LOQs ranging from 0.9 to 4 µg/ml (5 to 32 ng).

To learn more about the specifics of this research, including detailed findings and recommendations for optimizing your LNP analysis, access the full application note.