Development And Optimization Of LNP Formulations Using The KNAUER NanoScaler

The rise of lipid nanoparticles (LNPs) as a delivery platform for nucleic acid-based therapeutics has been fueled by the success of SARS-CoV-2 vaccines. Achieving scalable, efficient, and reproducible LNP production remains critical for clinical and commercial applications. This document explores the capabilities of KNAUER’s NanoScaler, a benchtop Impingement Jets Mixing (IJM) system designed for LNP formulation. By precisely controlling operating parameters such as total flow rate (TFR) and aqueous-to-organic phase ratio, the NanoScaler enables the production of LNPs with optimal size, polydispersity index (PdI), and encapsulation efficiency.

Our study details how variations in TFR and payload types influence LNP characteristics and demonstrates the seamless scalability from research to production scales. Results highlight the NanoScaler's superior performance in producing high-quality LNPs with consistent results across scales and payloads, including mRNA and pDNA. In-line dilution and efficient cleaning protocols further establish the system's utility for modern manufacturing environments.

Access the full content to explore how the NanoScaler can revolutionize your LNP development pipeline.