RNA Whole Transcriptome Sequencing From Challenging Matrices: Insights From Tissue

Biomarker discovery and translational research depend on reliable sequencing data, but extracting high-quality RNA from challenging tissue samples can be difficult. Skin, gut, and tumor biopsies each present obstacles that can affect RNA integrity, yield, and downstream analysis. Developing extraction workflows that account for these challenges is essential to generating consistent data and supporting robust sequencing results.

This webinar explores a systematic approach to RNA extraction protocol development, beginning with porcine skin biopsies before extending validation to human skin, gut, and tumor samples. The presentation examines how sample preservation influences RNA integrity, how extraction methods can be adapted for low-quality or degraded material, and how upstream decisions affect library preparation and sequencing performance. By following the workflow from initial development through validation across multiple tissue types, the session highlights practical considerations for improving RNA recovery and quality when working with difficult biological samples. It also explores factors researchers should consider when refining extraction protocols to meet the requirements of their specific applications.

For scientists working in biomarker discovery and translational research, these insights can help inform method selection and workflow optimization. Watch the webinar below to learn how the approach was developed, tested, and evaluated across challenging tissue types.