DNA oligonucleotides obtained by solid-phase synthesis contain incomplete or erroneous sequences that require removal for the advancement of these molecules as therapeutics. These key impurities in crude samples are length-based and are best removed by anion exchange chromatography. In this webcast, data is presented for the purification of two oligonucleotides (20-mer and 21-mer) having a phosphodiester backbone using Nuvia HP-Q Resin, a high-performance strong anion exchanger. Both yield (~90%) and purity (~98%) data were obtained during scale-up studies and are discussed. In addition, the data indicates that this strong anion exchange resin can also be used for the efficient purification of fully thiolated oligonucleotides used in clinical trials.
- Impurities are best removed by strong anion exchange chromatography
- Key process parameters were optimized to increase yield and purity
- A cost-effective, efficient, and scalable purification method was achieved