Preparative Anion Exchange Chromatography For The Purification Of DNA Oligonucleotides

DNA oligonucleotides obtained by solid-phase synthesis contain incomplete or erroneous sequences that require removal for the advancement of these molecules as therapeutics. DNA oligonucleotides have a high degree of negative charge and therefore can be separated according to length with anion exchange chromatography.

Here, we present the characterization of the high-performance strong anion exchange resin Nuvia HP-Q for the purification of single-stranded DNA oligonucleotides. Developed for the industrial downstream purification of large biomolecules at fast flow rates, Nuvia HP-Q Resin has technical characteristics such as bead size, pH stability, and flow characteristics that make the resin a promising candidate for ON purification. Key variables we evaluated include pH, flow rate, dynamic binding capacity, gradient slope, and scalability.