pDNA Lipid Nanoparticle Formulation And Screening Using Nova IJM

By Ryan Markewicz, Elizabeth Klavon, M.S., Canaan Pratt, Graham Taylor, PhD.

DNA Lipid Nanoparticles, or DNA+LNPs, are used extensively in gene editing and gene therapy as researchers explore their potential applications in a wide range of diseases and conditions. To address limitations in the availability of proven LNP manufacturing techniques, Helix Biotech has developed the novel Nova Benchtop (BT) platform. Review the performance of this platform when evaluating the effects of formulation and process parameters on the characteristics of DNA+LNP nanoparticles. Some of the changes that were analyzed include the type of ionizable lipid, PEG content, N/P ratio, and lipid stock concentration. This demonstration is intended to show how the Nova BT platform can enable researchers to more effectively develop and refine their formulations, potentially leading to more successful clinical outcomes.