Optimization And Scale-Up Of A Plasmid DNA Production Process

Plasmid DNA (pDNA) production plays a foundational role in gene therapy, vaccine development, and other genetic engineering applications—but scaling up the process efficiently remains a challenge. This application note explores a systematic approach to optimizing pDNA manufacturing in E. coli, beginning with process parameter screening and feasibility fermenter runs, and culminating in successful scale-up. Using a design of experiments (DOE) methodology, researchers identified TB media supplemented with feed and glycerol as the ideal conditions for maximizing yield in One Shot Top10 E. coli. The findings offer practical insights for bioprocess engineers and upstream development teams looking to improve productivity and streamline scale-up strategies.

For teams working on therapeutic or vaccine platforms, discover how this data-driven approach to pDNA production can help accelerate timelines and reduce variability.