Match The Polymerase To The Workflow

Capping strategy and promoter compatibility determine how RNA polymerase enzymes perform in IVT reactions, and not every enzyme suits every template design. This video walks through three Primrose Bio enzymes, PrimaCap AU, PrimaCap AG, and Prima7 Pro, each built around a distinct capping or promoter priority, from AU-capped and self-amplifying RNA strategies to existing T7 promoter workflows that need no redesign.

This video discusses capping efficiency, double-stranded RNA levels, and cumulative immunogenicity as factors separating these options, without spelling out how each one measures up head to head. Watch the video to explore how these three enzymes compare.