Levers To Optimize The IVT Reaction For Increased mRNA Yield

In vitro synthesis of single-stranded mRNA molecules is a widely used laboratory procedure that is central to RNA research and therapeutic development. Individual constructs and intended uses require a tailored manufacturing process. It is therefore important to know the critical factors that influence the in vitro transcription (IVT) reaction.

Read through this summary of some tools that can be used to set up and optimize the IVT reaction for enhanced mRNA yield, along with a demonstration that factors such as magnesium ion and nucleotide concentrations significantly impact IVT reaction performance and the overall yield of mRNA .