LC/MS/MS Vs. LC/HRMS: Identifying And Quantifying Oligonucleotides

Oligonucleotides (OGNTs) are short, single- or double-stranded DNA or RNA molecules that serve a wide range of functions in molecular biology, offering innovative approaches to targeting diseases at the molecular level. As OGNT-based therapeutics continue to gain traction in drug development, precise identification and quantification are essential for ensuring their efficacy and safety.

Two advanced mass spectrometry techniques commonly used for OGNT analysis are LC/MS/MS and LC/HRMS, each offering distinct advantages depending on the analytical needs of a project. Selecting the most suitable approach depends on several factors, including the available instrumentation, the specific goals of the study, and the level of detail required from a single injection. By carefully evaluating these considerations, researchers can optimize their analytical strategies to support successful drug development.