ExTend Cap AU Kit: Next-Gen Polymerase For Self-Amplifying mRNA

Maximizing the quality of self-amplifying mRNA (saRNA) often involves a difficult trade-off between high yields and the presence of immunogenic impurities. Traditional T7 polymerases frequently produce high levels of double-stranded RNA (dsRNA), which increases the complexity of downstream purification and can impact the safety profile of mRNA therapeutics.

The Prima RNApols ExTend Cap AU kit addresses these manufacturing bottlenecks by delivering ultra-low dsRNA levels while maintaining superior transcript integrity and yield. Specifically designed for AU capping, this polymerase achieves >95% efficiency using lower concentrations of cap analogs and enzymes. These improvements allow for a more cost-effective manufacturing process without sacrificing performance. You can review the full performance data to see how this next-generation polymerase compares to standard and mutant T7 options.