Case Study: Exploring The Spatial Distribution Of mRNA-Lipid Nanoparticles In Mouse Whole-Body And Isolated Organs Using MALDI MSI

This study reveals the extended persistence and broad organ distribution of LNP1 in mice — particularly in the liver and spleen — while uncovering notable gender-specific pharmacokinetic differences that warrant deeper exploration. In contrast, the lack of detectable LNP2 in female subjects highlights potential sensitivity challenges with MALDI-MSI for certain lipid nanoparticle classes.

These findings underscore the critical role of high-resolution MALDI-MSI not only in mapping drug biodistribution but also in characterizing the pharmacokinetics and tissue-level localization of advanced drug delivery systems. By visualizing spatial heterogeneity within organs and aligning it with histological features, this approach enhances our understanding of toxicity and therapeutic targeting.

Explore the full poster to see how these insights could refine preclinical evaluation strategies and advance lipid nanoparticle-based therapies.