Best Practices For Analysis Of In Vitro Transcribed (IVT) mRNA: Using The Agilent Fragment Analyzer Systems

The development of mRNA therapeutics and vaccines relies on reliable and precise methods for analyzing in vitro transcribed (IVT) RNA. Due to its large size and inherent heterogeneity, accurately assessing the critical quality attributes (CQAs) of IVT RNA presents significant analytical challenges.

This overview provides best practices for improving the resolution, accuracy, and precision of IVT RNA analysis using automated capillary electrophoresis (CE). Key recommendations cover sample preparation, including the proper dilution and denaturation of the IVT RNA sample, to prevent aggregation and ensure accurate data. It also details best practices for maintaining the capillary electrophoresis system, such as using specialized kits and methods optimized for IVT RNA, to achieve high-resolution separation and reliable quantification of the main product and impurities. These practices are vital for advancing the robust development and quality control of mRNA-based drugs.

Access the full application note for detailed guidance on optimizing your IVT RNA analysis workflow.