Advancing mRNA Therapeutics With High Performance RNA Polymerases

By Maite Arrizubieta, Patrik Engström, Shawn Walsh, Christopher Schvarcz, Malithi Jayasinghe, Page Daniels, Nick Halloran, Barbara Frey, Sabrina Baffert, Daniel Olson, Khaled Yamout and Helge Zieler, Primrose Bio

Advanced enzyme engineering has paved the way for a new generation of RNA polymerases designed to overcome these hurdles in manufacturing efficiency and transcript purity by optimizing multiple key performance indicators simultaneously. These engineered enzymes demonstrate significant improvements in yield and capping efficiency while drastically reducing the formation of double-stranded RNA, a common and often problematic byproduct.

By enhancing the utilization of DNA templates, these tools offer a more cost-effective and scalable pathway for developing complex therapeutics and vaccines. Explore the data behind these performance improvements and see how refined enzymatic performance can streamline your therapeutic production.