Accelerate Your pDNA And mRNA Process Development

Plasmid DNA (pDNA) production plays a pivotal role in gene therapy, vaccine development, and genetic engineering, but scaling up this process without compromising yield or quality remains a challenge. This webinar outlines a structured, three-phase approach to optimizing and scaling pDNA production using E. coli OneShot Top10. Starting with a 4-factor, 2-level design of experiments (DOE), the team identifies key parameters—vessel type, media selection, feed supplementation, and carbon source—that influence culture growth and pDNA yield. These optimized conditions are validated in a 1 L fermentor before being scaled to a 30 L Xcellerex™ XDR-50 system.

Explore the results that demonstrate consistent performance and yield across scales, offering a reliable framework for upstream process development.