A Multiplexed High Throughput Method To Evaluate Novel Nuclease Activity

Advancements in genome editing have expanded far beyond the original CRISPR-Cas9 system, with novel nucleases offering enhanced precision, flexibility, and efficiency. However, evaluating these nucleases in a high-throughput, comparative manner remains a significant challenge due to variations in PAM sequence requirements and editing mechanisms.

This application note introduces a streamlined, nuclease-agnostic workflow that enables rapid, multiplexed assessment of novel nucleases. By leveraging Synthego’s Halo™ Platform for synthetic sgRNA synthesis and automation, combined with Paragon Genomics’ CleanPlex^® amplicon sequencing technology, researchers can efficiently generate and analyze NGS-ready libraries. This method eliminates the need for custom target redesign, supports rapid evaluation across multiple genomic loci, and reduces hands-on time through automation.

Learn how this approach simplifies nuclease evaluation, ensuring accurate side-by-side comparisons while accelerating gene-editing advancements. Read on to explore the full methodology and its potential impact on genome engineering.